Microscope calibration can help ensure that the same sample, when assessed with different microscopes, will yield the same results. Even two identical microscopes can have slightly different magnification factors when not calibrated.
The scale on the eyepiece reticle does not have units and the values change at different magnifications. Therefore, it is important to calibrate the eyepiece reticle before taking measurements to ensure that the microscope will output accurate and valid information.
Let Us Care For & Calibrate Your Microscope
LTI Metrology, near Philadelphia, PA, has a field calibration team to provide microscope calibration services on-site at our customers’ facilities. They have the expertise to calibrate microscope reticles with the reliability you need. Routine cleaning and maintenance are also offered, with or without the calibration service.
Our qualifications attest to our reliability:
- Microscope calibration is A2LA accredited and NIST traceable
- Calibration services have been a specialty of LTI for nearly 30 years
- Calibration Certificates will meet your quality requirements with accreditation and traceability information, “as found” and “as left” data, and detailed results
LTI Metrology is a full-service laboratory with the expertise and equipment to calibrate all types of masters, measuring hand tools and measuring equipment in our lab or on-site.
Request a quote or schedule on-site calibration services at 215-997-9103 or firstname.lastname@example.org.
LTI Metrology Capabilities
- Calibrating most microscope models
- Cleaning and maintenance
- NIST traceability
- A2LA accreditation to ISO/IEC 17025
The Microscope Calibration Process
The size of objects in the microscope’s field of view differs at each magnification, so the actual diameters of these fields of view need to be calculated through calibration. The process of microscope calibration is a comparison of the grid on the eyepiece reticle with a stage micrometer showing units in millimeters or micrometers to validate the scale. When calibrating, the stage micrometer is lined up with the grid on the reticle, then the number of divisions on the microscope are counted per millimeter or micrometer on the staged micrometer. The number of divisions will change as the magnification changes.