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Microscope Calibration

Microscope CalibrationMicroscope calibration is important to ensure that the same sample, when assessed with different microscopes, will yield the same results. Even two identical microscopes can have slightly different magnification factors when not calibrated. The scale on the eyepiece reticle does not have units and the values change at different magnifications. Therefore, it is important to calibrate the eyepiece reticle before taking measurements to ensure that the microscope will output accurate and valid information.

The field calibration team at LTI Metrology near Philadelphia, PA will calibrate microscope reticles on-site for customers at their facilities. Routine cleaning and maintenance is also offered with or without microscope calibration services.

Microscope calibration is A2LA accredited and NIST traceable. Calibration Certificates include accreditation and traceability information, in addition to “as found” and “as left” data and detailed calibration results. LTI Metrology is a full-service laboratory with the expertise and equipment to calibrate all types of masters, measuring hand tools and measuring equipment in our lab or on-site. Request a quote or schedule on-site calibration services at 215-997-9103 or email

The Microscope Calibration Process

The size of objects in the microscope’s field of view differs at each magnification, so the actual diameters of these fields of view need to be calculated through calibration. The process of microscope calibration is a comparison of the grid on the eyepiece reticle with a stage micrometer showing units in millimeters or micrometers to validate the scale. When calibrating, the stage micrometer is lined up with the grid on the reticle, then the number of divisions on the microscope are counted per millimeter or micrometer on the staged micrometer. The number of divisions will change as the magnification changes.

LTI Metrology Capabilities

  • Calibrating most microscope models
  • Cleaning and maintenance
  • NIST traceability
  • A2LA accreditation to ISO/IEC 17025